Ross Imaging Center

Overview of Services

The Ross Fluorescence Imaging Center is available to all JHU researchers, and outside users, with our main focus being the members of our NIH NIDDK funded P30 Center and collaborators in the Division of Gastroenterology. We have additional interactions with members of the Division of Cardiology, throughout Department of Medicine, and School of Medicine. We collaborate closely with the Department of Anaesthesiology and Critical Care Medicine (ACCM), managing their confocal microscope core as a separate iLab core (ACCM Confocal Microscope).

 The DCC’s areas of research emphasized include Epithelial transporter function and regulation (with emphasis on trafficking, protein-protein interactions and heavy metal transport), Inflammation, injury and fibrosis, Neurogastroenterology, and GI pre-cancer (with emphasis on signaling pathways, inflammation, epigenetics, and biomarkers).

 The mission of Hopkins DDC Imaging Core B is to provide equipment and service of classical and cutting-edge light microscopy and spectroscopy techniques to our Research Base investigators for study of the physiology and cell biology of the GI track at the level of organs, tissues, cell models, co-cultures, sub-cellular organelles and single molecules. These approaches include wide-field, confocal and multiphoton microscopy with applications for immunofluorescence; single molecule RNA fluorescence in situ hybridization (smFISH); measurements of intracellular pH, Ca2+and other ions by small molecule indicators and fluorescent protein biosensors; methods to determine protein trafficking at a single cell level; FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models; FRAP microscopy for monitoring the changes in protein mobility and binding in protein complexes; and multicolor spectral imaging with more than 4 fluorophores.

 We are located in the Ross Research Building 9th floor (service corridor S913, S910, S972). Our major focus is confocal microscopy, with an Olympus FV3000RS inverted confocal microscope. We also manage ACCM’s Leica SP8 confocal microscope. Our new single molecule fluorescence in situ hybridization microscope (“FISHscope”) is a state of the art 5plex microscope, complemented by development of smFISH probe sets by our core director, Prof. Bin Wu. We have an Olympus FV1000MPE multiphoton microscope, Keyence BZ-X700 “box” microscope, Zeiss inverted microscope with Olympus DP80 dual monochrome & RGB CCDs camera, multiple MetaMorph Imaging System software licenses, and expert fluorescence microscopy consultations and training from Image Core Manager George McNamara, PhD, and Image Core Director Prof. Bin Wu, PhD.

 We additionally manage the iLab schedulers for several additional instruments, including Li-Cor Odyssey CLx Western blot scanner, PerkinElmer EnVision 2104 multi-mode plate reader (absorbance, fluorescence, luminescence, Alpha screen), and two Horiba PTI-fluorimeters.

Leadership

Bin Wu, PhD | Director

George McNamara, PhD | Manager

Location and hours of operation

Hours Location

Training: Monday - Friday 9 AM - 5 PM

Instrumentation is available 24/7 to fully

    trained users (scan card or key access)    

Ross Research Bldg., 9th floor

720 Rutland Ave, Baltimore, MD 21205-2195

Rooms: S913, S910, S972 (service corridor)

 

Web links

 

Contacts

Name Role Phone Email Location
George McNamara
Manager
 
305-764-2081
 
gmcnamara@jhmi.edu
 
Ross S913