Ross Imaging Center

Overview of Services

The Ross Fluorescence Imaging Center is available to all JHU researchers, and outside users, with our main focus being the members of our NIH NIDDK funded P30 Center and collaborators in the Division of Gastroenterology ("GI Center"). We have additional interactions with members of the Division of Cardiology, throughout Department of Medicine, and School of Medicine. We collaborate closely with the Department of Anaesthesiology and Critical Care Medicine (ACCM), managing their confocal microscope core as a separate iLab core (ACCM Confocal Microscope).

 The DCC’s areas of research emphasized include Epithelial transporter function and regulation (with emphasis on trafficking, protein-protein interactions and heavy metal transport), Inflammation, injury and fibrosis, Neurogastroenterology, and GI pre-cancer (with emphasis on signaling pathways, inflammation, epigenetics, and biomarkers).

 The mission of Hopkins DDC Imaging Core B is to provide equipment and service of classical and cutting-edge light microscopy and spectroscopy techniques to our Research Base investigators for study of the physiology and cell biology of the GI track at the level of organs, tissues, cell models, co-cultures, sub-cellular organelles and single molecules. These approaches include wide-field, confocal and multiphoton microscopy with applications for immunofluorescence; single molecule RNA fluorescence in situ hybridization (smFISH); measurements of intracellular pH, Ca2+and other ions by small molecule indicators and fluorescent protein biosensors; methods to determine protein trafficking at a single cell level; FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models; FRAP microscopy for monitoring the changes in protein mobility and binding in protein complexes; and multicolor spectral imaging with more than 4 fluorophores.

 We are located in the Ross Research Building 9th floor (service corridor S913, S910, S972). Our major focus is confocal microscopy, with an Olympus FV3000RS inverted confocal microscope. We also manage ACCM’s Leica SP8 confocal microscope. Our new single molecule fluorescence in situ hybridization microscope (“FISHscope”) is a state of the art 5plex microscope, complemented by development of smFISH probe sets by our core director, Prof. Bin Wu. We have an Olympus FV1000MPE multiphoton microscope, Keyence BZ-X700 “box” microscope, Zeiss inverted microscope with Olympus DP80 dual monochrome & RGB CCDs camera, multiple MetaMorph Imaging System software licenses, and expert fluorescence microscopy consultations and training from Image Core Manager George McNamara, PhD, and Image Core Director Prof. Bin Wu, PhD.

 We additionally manage the iLab schedulers for several additional instruments, including Li-Cor Odyssey CLx Western blot scanner, PerkinElmer EnVision 2104 multi-mode plate reader (absorbance, fluorescence, luminescence, Alpha screen), and two Horiba PTI-fluorimeters.

Leadership

Bin Wu, PhD | Director

George McNamara, PhD | Manager

Location and hours of operation

Hours Location

Training: Monday - Friday 9 AM - 5 PM

Instrumentation is available 24/7 to fully

    trained users (scan card or key access)    

Ross Research Bldg., 9th floor

720 Rutland Ave, Baltimore, MD 21205-2195

Rooms: S913, S910, S972 (service corridor)

 

Web links

 

20210126Tue: Services - use Schedule Equipment tab ( https://johnshopkins.corefacilities.org/service_center/5148/?tab=equipment ) to make reservations. When you use any equipment, enter your name and start time (and date) "first thing", and then sign out when done. Failure to sign in at start of each session means you are trespassing on JHU property. Billing of our confocal microscopes is in 1/2 hour interval (rounded up) from sign-in sheet. Other equipment is billed by reservation. 

Consultation ... with George McNamara, PhD (arrange meeting by email first, gmcnamara@jhmi.edu)
Olympus FV3000RS ... confocal microscope
Zeiss AxioObserver.A1 inverted microscope w/DP80 CCD
Keyence BZ-X710 all-in-one microscope
Olympus IX83 Inverted Microscope ("FISHscope")
Li-Cor Odyssey CLx Western blot scanner
Biotek absorbance plate reader (Ross S972) - all users must sign in
PTI Quantamaster 40 Fluorimeter #1 Ross S913
PTI Quantamaster 40 Fluorimeter #2 Ross S972
MetaMorph image analysis #1
MetaMorph image analysis #2
Olympus FV1000MPE Multiphoton Microscope

VueScan slide scanner ... this service is in preliminary phase, similar image quality as https://works.bepress.com/gmcnamara/1/ 

See also http://confocal.jhu.edu/current-equipment/

 Note: four of our microscopes can "do" tile scanning and image stitching AND/OR automate stage relocation for "cyclic" imaging (ex 12plex RNAscope 3 cycles of 4 probes). This can enable more efficient acquisition at optimum spatial resolution (i.e.high NA objective lens, wavelength limited pixel and Z-step size) (if the analysis computer can handle our 'data deluge') without you being present the whole time (a major benefit of automated microscopy):

  * ACCM Leica SP8 confocal microscope (ACCM core in iLab; standard tile scan or NAVIGATOR 'app' wizard)

  * Olympus FV3000RS confocal

  * FISHscope (Olympus IX83)

  * Keyence BZ-X710

Our Olympus FV1000MPE multiphoton microscope can also tile scan -- a user 'did' a 50 slide project of kidney tissue fibrosis in 2018 -- but currently (1/2021) very nfrequently used microscope (we'd be happy to see more users on it).

1/2021: we have also put on VueScan slide scanner for histology / brightfield IHC slides onto a PC in the image core and an iLab scheduler here. This produces images equivalent to a 4x or 5x objective lens 'nominal resolution' (though sometimes the slide scanner focus is not quite as good). Nice for acquiring large area quickley, and then making measurements of areas in Fiji ImageJ, MetaMorph, Photoshop, etc. If users find it useful, great; if not, we may remove it as a service and just have it at GM's PC. Scanner is a repurposed 35 mm film scanner. You are welcome to scan in your 35 mm film 'cassettes' or film strips, when VueScan is not being used for research.

Leica SP8 confocal microscope is at https://johnshopkins.corefacilities.org/schedules/345585 
 
Reminder: all users must have an iLab reservation AND sign-in and out of the usage sheet. Failure to do is trespassing on JHU property.

 

Contacts

Name Role Phone Email Location
George McNamara, PhD
Manager
 
305-764-2081
 
gmcnamara@jhmi.edu
 
Ross S913