Welcome!
The Cardiovascular Physiology and Surgery Core is available to provide the Johns Hopkins community and beyond three types of cardiovascular procedures on small animals, including, but not limited to, mouse and rat: echocardiography, survival surgery, and hemodynamics. Other procedures may be possible after discussion with the core surgeons. Within each of these three areas, there are several different specific techniques and procedures that will be offered. Echocardiograpy: Functional assessment of the heart and major blood vessels is provided by this service. Images are generated using one of two systems -- an Accuson Sequioa echo-Doppler system or a state-of-the-art Visual-Sonics 2100 imaging system. The first is useful for routine M-mode and 2-D imaging, and for flow Doppler assessments. The latter provides higher resolution imaging and also has tissue Doppler capabilities, including speckle tracking for regional strain analysis. Functional imaging is also available to assess aortic diameter and wall thickening, flow in the aorta to assess vascular stiffening (pulse wave velocity, velocity time integral used to determine cardiac output, etc.). Survival Surgery: We provide a variety of surgically generated heart disease models for use in mice or rats. This includes aortic or pulmonary artery banding for pressure-overload, myocardial infarction or ischemic reperfusion models of the left ventricle, and osmotic pump implantation for delivery of pharmaceuticals. Pressure-Volume Loops: Analysis of right or left heart function is possible by inserting a miniature pressure-volume catheter into the relevant ventricle to measure in situ cardiac function using pressure-volume relationships. This is used to assess ventricular pressures, systolic and diastolic function, relaxation rates, filling rates, load-independent contractility paramenters, disatolic compliance, and other indexes. These studies are not designed to be survival surgeries. Chronic Electrocardiographic Telemetry: Electrocardiographic recordings from implantable transmitters for multiple days, if needed, are available via this service.
Dear Users and PIs,
The Core will reopen on Monday, June 15th. Based on the JHU Research Return to Campus: Phase I Guidance, only low-risk activities will be allowed in Phase 1. Scheduling users and maintaining occupancy rate maximums will be essential.
The echo data analysis station in Ross 867 remains closed.
Scheduling and Reservations. All reservations booked before March 18th were cancelled with JHU’s closing. Please email us your new plans so we can try to organize a re-start that will optimize use and maximize safety. However, please note that any reservations will be tentative and can be cancelled at any time by Core staff, or if JHU SOM shuts down again.
Safety Precautions. Everyone should do their best to practice good safety procedures for their own health and for the health of those around them. Please do your part to maximize the safety of all of us during COVID-19 pandemic. To this end, please follow the JHU SOM Phase 1 Guidance. If you are sick, or have been in recent contact with someone who showed COVID-19 infection symptoms, you should not come to your session, which can be cancelled without charge.
While performing laboratory activities at the Core laboratories:
We will try to arrange for 70% EtOH spray bottles, hospital-grade disinfectant spray bottles and paper towels for every work area. Core staff will clean the laboratory surfaces daily using EPA-approved hospital-grade disinfectant.
Any Hazardous Biological, Chemical, Radioactive. Please inform the Core staff before any experiment if you have used hazardous chemicals, biologicals (especially pathogenic virus, bacteria) or radioactive materials on your animals.
If you have any or concerns, please email us.
David Kass, Professor Program Chairman
410-955-7153
dkass@jhmi.edu
Nadan Wang, Lab Manager/Director
410-502-2044
nwang31@jhmi.edu
Michelle "Missy" Leppo, Surgical Technician
410-955-3814
mleppo@jhmi.edu
Shi Pan, Research Associate
410-502-2044
span24@jhmi.edu
|
Nadan Missy Shi |
720 Rutland Avenue. Baltimore, MD 21205 720 Rutland Avenue, Baltimore, MD 21205 720 Rutland Avenue. Baltimore, MD 21205 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Michelle "Missy" Leppo |
Surgical Technician
|
410-955-3814
|
mleppo@jhmi.edu
|
Ross 812
|
| Nadan Wang |
Director
|
410-502-2044
|
nwang31@jhmi.edu
|
Ross 868
|
| David Kass |
Program Chairman
|
410-955-7153
|
dkass@jhmi.edu
|
Ross 858
|
| Shi Pan |
Research Associate
|
410-502-2044
|
span24@jhmi.edu
|
Ross 868
|
