About

Eukaryotic Tissue Culture Facility

Overview of Services

The Eukaryotic Tissue Culture facility supports JHMI researchers with the culturing of insect and mammalian cells. The facility operates with two service models: (1) a ‘assisted’ model, where facility staff carry out cell culture and expression for users, and (2) an ‘independent’ model, in which JHMI researchers use facility instrumentation. The facility is designed to provide JHMI researchers with space and equipment for eukaryotic expression and for training laboratory personnel, thereby avoiding a need for individual labs to set up their facilities for eukaryotic cell growth and protein expression.

 

The facility provides:
• Eukaryotic protein expression in BSL-2 tissue culture laboratory
• Laboratory equipment for insect cell and mammalian cell culture, including tissue culture hoods, refrigerators, chilled centrifuges, incubators, liquid nitrogen storage, microscope, cell counter, Centrimate tangential flow system.

Leadership

Sandra B. Gabelli
Co-Director
Phone: 410-614-4145
Email:gabelli@jhmi.edu

 

James Berger
Co-Director
Email: jmberger@jhmi.edu

 

Yana Li
Facility Manager
Phone: 443-287-1995
Email: yli123@jhmi.edu

Location and hours of operation

Hours Location

Monday - Friday 9:00am - 5:00pm 

Biophysics 306
725 N Wolfe St
Baltimore, MD 21205

Hunterian 708-710
725 N Wolfe St
Baltimore, MD 21205

 

Publications that used ETCF

  1. Kalin, J., Wu, M., Gomez, A., Song, Y., Das, J., Hayward, D., Adejola, N., Panova, I., Chung, H. J., Kim, E., Roberts, H. J., Roberts, J. M., Prusevich, P., Jeliazkov, J. R., Roy Burman, S.S., Fairall, L., Milano, C., Eroglu, A., Proby, C., Dinkova-Kostova, A. T., Hancock, W. W., Gray, J. J., Bradner, J. E., Valente, S., Mai, A., Anders, N. M., Rudek, M. A., Ryu, B., Schwabe, J. W. R., Mattevi, A., Alani, R. M., Cole, P. A. (2018) Targeting the CoREST Complex with Dual Histone Deacetylase and Demethylase Inhibitors Nature Communications 9:53.
  2. Wu, M., Hayward, D., Kalin, J. H., Song, Y., Schwabe, J.W.R., Cole, P.A. (2018) Lysine-14 Acetylation of Histone H3 in Chromatin Confers Resistance to the Deacetylase and Demethylase Activities of an Epigenetic Silencing Complex eLife in press.
  3. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Venkataraman A, Yang K, Irizarry J, Mackiewicz M, Mita P, Kuang Z, Xue L, Ghosh D, Liu S, Ramos P, Hu S, Bayron Kain D, Keegan S, Saul R, Colantonio S, Zhang H, Behn FP, Song G, Albino E, Asencio L, Ramos L, Lugo L, Morell G, Rivera J, Ruiz K, Almodovar R, Nazario L, Murphy K, Vargas I, Rivera-Pacheco ZA, Rosa C, Vargas M, McDade J, Clark BS, Yoo S, Khambadkone SG, de Melo J, Stevanovic M, Jiang L, Li Y, Yap WY, Jones B, Tandon A, Campbell E, Montelione GT, Anderson S, Myers RM, Boeke JD, Fenyö D, Whiteley G, Bader JS, Pino I, Eichinger DJ, Zhu H, Blackshaw S. Nat Methods. 2018 Mar 19. doi: 10.1038/nmeth.4632.
  4. Sweta Maheshwari, Michelle S. Miller, Robert O'Meally¶, Robert N. Cole, L. Mario Amzel and Sandra B. Gabelli. JBC 292, 13541-13550
  5. Chen Z1, Jiang H1, Xu W1, Li X2, Dempsey DR1, Zhang X3, Devreotes P2, Wolberger C, Amzel LM, Gabelli SB, Cole PA.A Tunable Brake for HECT Ubiquitin Ligases.Mol Cell. 2017 May 4;66(3):345-357.e6. doi: 10.1016/j.molcel.2017.03.020. .

 

Links and Resources

  1. Cell Culture Basics from Gibco-Invitrogen (animation)

Contacts

Name Role Phone Email Location

Service list


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