Mass Spectrometry and Proteomics Core

Overview of Services

The Johns Hopkins University (JHU) School of Medicine (SOM) Mass Spectrometry and Proteomics Facility assists investigators to identify and quantify proteins and their modifications that are differentially expressed in cells, tissues or body fluids, to track interactions with binding partners during changes in signal transduction, and to identify proteolytic cleavage sites and map post-translational modifications by providing the following proteomic services:

  • Consultation: Pre- and post-analysis.
  • Sample preparation: Buffer exchange; Column chromatography; Proteolytic digestion; Stable isotope labeling.
  • Protein Identification: Proteins in solution, in gel bands or spots, or in complex protein extracts.
  • Protein Modifications: Acetylation, AMPylation, Citrullination, Glycosylation, Phosphorylation, Proline hydroxylation, Nitrosation, Ubiquitination, Novel cleavage sites, etc.
  • Protein Quantification: Relative quantification using isobaric mass tags (TMT, iTRAQ) or stable isotopic amino acids (SILAC); Targeted (absolute) quantification using standard peptides (AQUA) or recombinant proteins (PSAQ).
  • High Resolution Mass Analysis: Accurate mass of intact proteins; Characterization protein modifications.
  • Biostatical/Bioinformatic Analysis: PCA analysis, Volcano plots, Ingenuity based gene ontology pathway analysis; Bioinformatics consultation.
  • Training and Self-Service Equipment: In addition, the Core offers workshops to use the Core’s MALDI-TOF mass spectrometer, Typhoon gel imaging and 2D gel electrophoresis equipment.

Location and hours of operation

Hours Location

 9 am - 5 pm

Monday - Friday 

Wood Basic Science Bldg (WBSB) Room 503

725 N. Wolfe Street

Baltimore, MD 21205

Drop Off Samples between 10 am - 4 pm

Links and Resources

  1. Mass Spectrometry and Proteomics Facility
  2. Scaffold Viewer
  3. Proteome Discoverer Viewer
  4. Peaks Viewer
  5. Cancer Center Member List (SKCCC)
  6. GI Center Member List (DDBRDC)

Leadership

Robert N. Cole, Ph.D.

Director

410-614-6968 

rcole@jhmi.edu

Staff

Tatiana N. Boronina, Ph.D. Robert N. O'Meally, M.S. Lauren R. DeVine, M.S.
Proteomics Specialist Proteomics Specialist Proteomics Specialist
410-614-6968 410-614-6968 410-614-6968
tboroni1@jhmi.edu romeall1@jhmi.edu LaurenDeVine@jhmi.edu

 

 

Contacts

Name Role Phone Email Location
Robert N. Cole, PhD
Director
 
410-614-6968
 
rcole@jhmi.edu
 
MRB 371
 
Jackie Seidl
Adminstrative Assistant
 
410-955-2739
 
jaseidl@jhu.edu
 
WSBS 414
 

Services

Name Description

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