Transcriptomics and Deep Sequencing Core

 

Due to high demand and low staff, RUSH options will not be available through the remainder of the year as we are unable to process samples in a properly expedited fashion.  We apologize for any inconvenience.

 

NOVASEQ PRICE REDUCTION!

Effective December 1, 2020, the Core is excited to announce reduced costs on most NovaSeq runs!  Sequence more for less!  Combine our new lower NovaSeq prices with new lower Single Cell library prep costs and take advantage of huge discounts.

 

SINGLE CELL RNA-SEQ PRICING UPDATE!

Effective 10/6/2020, new lower prices for single-cell sequencing allows our customers to process more and save more!  Receive bulk discounts on as few as TWO samples!

 

6/10/20 NEW COVID-19 Update:

 

Effective Monday, June 15, the Transcriptomics Core will re-open!  In accordance with University policy, we will observe all social distancing requirements.  To ensure the safety of our clients and lab members, APPOINTMENTS ARE REQUIRED FOR SAMPLE SUBMISSION.  iLabs submission is required before scheduling an appointment.

 

Please go to the Schedule Equipment tab to reserve a time slot for sample submission.

 

 

 

 

Pricing update:

Effective 1/1/2020, the monthly usage charge for Ingenuity Pathway Analysis will increase to $300.  The hourly charge for computer usage will increase to $30/hour.

 

 

Overview of Services

Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principal investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April 2016). We offer sample preparation and analysis using Next Generation & Third Generation sequencing technologies, Microarrays, Nanostring nCounter, and qPCR.  We also offer high throughput DNA, Chromatin, and RNA shearing using the Covaris E220 system. Our lab is equipped with both PacBio and Oxford Nanopore platforms for third generation, long read, single-molecule real-time (SMRT) sequencing, as well as the Illumina NextSeq 500, MiSeq, and iSeq next-generation sequencer for short reads. We work closely with the ECGC to enable our clients access to the Illumina HiSeq 2500/3000 and NovaSeq for deeper sequencing requirements. The third-generation long-read sequencing technologies are great for genome assembly, novel transcript isoform identification, full-length 16s rRNA gene sequencing for metagenomics, repeat sequences, and GC-rich region targeted resequencing, as well as for bacterial DNA methylation study.  The Illumina short-read sequencing technology, with its high throughput, is great for whole-genome resequencing, whole-exome sequencing, RNAseq, ChIPseq, 16s rRNA gene, or whole-genome metagenomic sequencing, and targeted DNA resequencing.  We run the Affymetrix microarray system while offering preparation and support for Agilent arrays as well. In addition, we offer the Nanostring nCounter system for RNA expression, FFPE analysis, CNV, and miRNA detection. We ensure a full-service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one-stop-shop.  

 

Core Publications List

Leadership

Seth Blackshaw, Ph.D., Faculty Director
MRB Room 339
Email: sethblackshaw@gmail.com

 

Linda Orzolek, MS, Director
MRB Room 363
Office Phone: (410) 502-6658
Lab Phone: (443) 287-0262
Email: ldorsch2@jh.edu

Location and hours of operation

Hours Location

Hours Open: Monday - Friday 8:30am-5:00pm

Hours Fully Staffed: Monday - Friday 8:30am-5:00pm

733 N Broadway
MRB 360
Baltimore, MD 21205

Links and Resources

1. JHMI Transcriptomics and Deep Sequencing Core

Contacts

Name Role Phone Email Location
Linda D. Orzolek, MS
Director
 
410-502-6658
 
ldorsch2@jhmi.edu
 
MRB Room 363
 
Seth Blackshaw, PhD
Faculty Director
 

 
sethblackshaw@gmail.com
 
MRB Room 335
 
Jasmeet Sethi, PhD
Sr. Research Specialist
 
443-287-8852
 
jsethi1@jhmi.edu
 
MRB Room 355
 
Conover Talbot Jr., BS
Bioinformatician
 
410-614-0443
 
cct@jhmi.edu
 
MRB Room 353
 
Haiping Hao, PhD
Consulting Director
 
443-287-9056
 
hhao@jhmi.edu
 

 

Price list


Search available services: View: by category alphabetically
Data Analysis (3)
Microarrays (26)
Nanostring (3)
Nucleic Acid Extraction (7)
PacBio (4)
Quality Control (9)
RT PCR (1)
Scanning (3)
Sequencing (42)
Shearing (4)
Single Cell Sequencing (8)

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