ALERT

In Observance of U.S. Holiday, Independence Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Friday July 3rd, 2020. We will resume regular U.S. support hours on Monday July 6th, 2020. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.

Transcriptomics and Deep Sequencing Core

 

6/10/20 NEW COVID-19 Update:

 

Effective Monday, June 15, the Transcriptomics Core will re-open!  In accordance with University policy, we will observe all social distancing requirements.  To ensure the safety of our clients and lab members, APPOINTMENTS ARE REQUIRED FOR SAMPLE SUBMISSION.  iLabs submission is required before scheduling an appointment.

 

Please go to the Schedule Equipment tab to reserve a time slot for sample submission.

 

 

 

 

Pricing update:

Effective 1/1/2020, the monthly usage charge for Ingenuity Pathway Analysis will increase to $300.  The hourly charge for computer usage will increase to $30/hour.

 

 

Overview of Services

Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principle investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April, 2016). We offer sample preparation and analysis using Next Generation & Third Generation sequencing technologies, Microarrays, Nanostring nCounter, and qPCR.  We also offer high throughput DNA, Chromatin and RNA shearing using Covaris E220 system. Our lab is equiped with both PacBio and Oxford Nanopore platforms for third generation, long read, single-molecule real-time (SMRT) sequencing, as well as the Illumina NextSeq 500, MiSeq and iSeq next-generation sequencer for short reads. We work closely with the GRCF to enable our clients access to the Illumina HiSeq 2500/3000 and NovaSeq for deeper sequencing requirements. The third generation long read sequencing technologies are great for genome assembly, novel transcript isoform identification, full length 16s rRNA gene sequencing for metagenomics, repeat sequences and GC-rich region targeted resequencing, as well as for bacterial DNA methylation study.  The Illumina short read sequencing technology, with its high throughput, is great for whole genome resequencing, whole exome sequencing, RNAseq, ChIPseq, 16s rRNA gene or whole genome metagenomic sequencing, and targeted DNA resequencing.  We run the Affymetrix microarray system, while offering preparation and support for Agilent arrays as well. In addition, we offer the Nanostring nCounter system for RNA expression, FFPE analysis, CNV, and miRNA detection. We ensure a full service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one stop shop.  

 

Core Publications List

Leadership

Seth Blackshaw, Ph.D. Director
MRB Room 335
Email: sethblackshaw@gmail.com

 

Haiping Hao, Ph.D., Associate Director
MRB Room 359
Office Phone: (443) 287-9056
Lab Phone: (443) 287-0262
Email: hhao@jhmi.edu

Location and hours of operation

Hours Location

Hours Open: Monday - Friday 8:30am-5:00pm

Hours Fully Staffed: Monday - Friday 8:30am-5:00pm

733 N Broadway
MRB 360
Baltimore, MD 21205

Links and Resources

1. JHMI Transcriptomics and Deep Sequencing Core

Contacts

Name Role Phone Email Location
Haiping Hao, PhD
Associate Director
 
443-287-9056
 
hhao@jhmi.edu
 
MRB Room 359
 
Seth Blackshaw, PhD
Director
 

 
sethblackshaw@gmail.com
 
MRB Room 335
 
Linda D. Orzolek
Sr. Research Specialist II,Lab Manager
 
410-502-6658
 
ldorsch2@jhmi.edu
 
MRB Room 363
 
Jasmeet Sethi, PhD
Sr. Research Specialist
 
443-287-8852
 
jsethi1@jhmi.edu
 
MRB Room 355
 
Conover Talbot Jr., BS
Bioinformatician
 
410-614-0443
 
cct@jhmi.edu
 
MRB Room 353
 

Price list


Search available services: View: by category alphabetically
Data Analysis (3)
Microarrays (26)
Nanostring (3)
Nucleic Acid Extraction (7)
PacBio (4)
Quality Control (9)
RT PCR (1)
Scanning (3)
Sequencing (41)
Shearing (4)

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