Single Cell & Transcriptomics Core


The Single Cell & Transcriptomics Core will be CLOSED July 4 through July 6 for

sample submission.  Please mark your calendar now and plan accordingly!


Effective 01/06/2023


RUSH options will not be available


If you have upcoming deadlines, please contact Linda Orzolek directly


to see if your timing needs can be met. 


 Join our NEW Single Cell list serve! 


Email: with subject line: subscribe

SingleCell Firstname Lastname




Overview of Services

Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principal investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April 2016). We offer sample preparation and analysis using Next Generation & Third Generation sequencing technologies, Microarrays, Nanostring nCounter, and qPCR.  We also offer high throughput DNA, Chromatin, and RNA shearing using the Covaris E220 system. Our lab is equipped with both PacBio and Oxford Nanopore platforms for third generation, long read, single-molecule real-time (SMRT) sequencing, as well as the Illumina NextSeq 500, MiSeq, and iSeq next-generation sequencer for short reads. We work closely with the ECGC to enable our clients access to the Illumina HiSeq 2500/3000 and NovaSeq for deeper sequencing requirements. The third-generation long-read sequencing technologies are great for genome assembly, novel transcript isoform identification, full-length 16s rRNA gene sequencing for metagenomics, repeat sequences, and GC-rich region targeted resequencing, as well as for bacterial DNA methylation study.  The Illumina short-read sequencing technology, with its high throughput, is great for whole-genome resequencing, whole-exome sequencing, RNAseq, ChIPseq, 16s rRNA gene, or whole-genome metagenomic sequencing, and targeted DNA resequencing.  We run the Affymetrix microarray system while offering preparation and support for Agilent arrays as well. In addition, we offer the Nanostring nCounter system for RNA expression, FFPE analysis, CNV, and miRNA detection. We ensure a full-service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one-stop-shop.  


Core Publications List



Linda Orzolek, MS, Director
MRB Room 363
Office Phone: (410) 502-6658
Lab Phone: (443) 287-0262


Seth Blackshaw, Ph.D., Faculty Director
MRB Room 339


Location and hours of operation

Hours Location

Hours Open: Monday - Friday 8:30am-5:00pm

Hours Fully Staffed: Monday - Friday 8:30am-5:00pm

733 N Broadway
MRB 360
Baltimore, MD 21205

Links and Resources

1. Transcriptomics and Deep Sequencing Core (


Name Role Phone Email Location
Linda D. Orzolek, MS
MRB Room 359
Seth Blackshaw, PhD
Scientific Director
MRB Room 335
Jasmeet Sethi, PhD
Bulk Sequencing specialist
MRB Room 355
Conover Talbot Jr., BS
MRB Room 353
Tyler Creamer, PhD
Single Cell Sequencing Specialist (RNA)
(443) 287-9056
MRB Room 363
Jongseok Lee, PhD
Single Cell Sequencing Specialist (DNA)
MRB Room 360

Price list

Search available services: View: by category alphabetically
Data Analysis (3)
Library Prep (26)
Nanostring (3)
Nucleic Acid Extraction (7)
Quality Control (9)
Sequencing (19)
Shearing (4)
Single Cell Sequencing (32)