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Single Cell & Transcriptomics Core

 

 Single Cell Symposium 2021

 

Wednesday, September 22, 10am

 

Single Cell discounts will be available 10/15/21-11/15/21 (registration link below)

 

Agenda:

 

10:00- The Single Cell & Transcriptomics Core Linda Orzolek, director

 

10:30- 10X Genomics- Maximizing single cell insights with Multiomics

and High Throughputs assays- Brad Toms, M.S. Science & Technology

Advisor 10x Genomics

 

11:00- MissionBio- Unleashing the Power of Single-Cell Multi-Modal

Analysis to Advance Precision Medicine- Julia Gouffon,Translational

Applications Liason

 

11:30- Parse Biosciences- Single-Cell answers through Split-pool

Combinatorial Barcoding, Taking the Next Step Forward- Adam

Zoubeidi, Sr. Technical Sales Manager

 

12:00- Illumina- Resolving Single Cell Data Analysis and Storage

Bottlenecks using the Cloud- Dan Gheba, Executive Sequencing

Specialist

 

12:30- Partek- Partek® Flow®: point-and-click analysis of Single Cell

RNA-Seq data with data visualization and statistical analysis- Dr. Simit

Patel, Senior Field Applications Scientist

 

1:00- Determining the Future of Single Cell at Johns Hopkins

Loyal Goff, PhD, Assistant Professor, Johns Hopkins University

 

1:15- Q&A, Panel discussion

 

Zoom registration

 

The Transcriptomics & Deep Sequencing Core

 

is now

 

The Single Cell & Transcriptomics Core!

Over the next several weeks you will see changes to our iLabs page and website reflecting our new focus.

Transcriptomics and Deep Sequencing Core (hopkinsmedicine.org)

Effective September 1, new prices are in effect for some of our bulk sequencing services: 

Bulk discounts are now available on library preps with 8 samples or more. 

Lower prices for DNAseq

Lower prices for miRNAseq

Prices changes for 16S library preparation

Return of RUSH sequencing (added fee $50)

 

 

 

Overview of Services

Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principal investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April 2016). We offer sample preparation and analysis using Next Generation & Third Generation sequencing technologies, Microarrays, Nanostring nCounter, and qPCR.  We also offer high throughput DNA, Chromatin, and RNA shearing using the Covaris E220 system. Our lab is equipped with both PacBio and Oxford Nanopore platforms for third generation, long read, single-molecule real-time (SMRT) sequencing, as well as the Illumina NextSeq 500, MiSeq, and iSeq next-generation sequencer for short reads. We work closely with the ECGC to enable our clients access to the Illumina HiSeq 2500/3000 and NovaSeq for deeper sequencing requirements. The third-generation long-read sequencing technologies are great for genome assembly, novel transcript isoform identification, full-length 16s rRNA gene sequencing for metagenomics, repeat sequences, and GC-rich region targeted resequencing, as well as for bacterial DNA methylation study.  The Illumina short-read sequencing technology, with its high throughput, is great for whole-genome resequencing, whole-exome sequencing, RNAseq, ChIPseq, 16s rRNA gene, or whole-genome metagenomic sequencing, and targeted DNA resequencing.  We run the Affymetrix microarray system while offering preparation and support for Agilent arrays as well. In addition, we offer the Nanostring nCounter system for RNA expression, FFPE analysis, CNV, and miRNA detection. We ensure a full-service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one-stop-shop.  

 

Core Publications List

Leadership

 

Linda Orzolek, MS, Director
MRB Room 363
Office Phone: (410) 502-6658
Lab Phone: (443) 287-0262
Email: ldorsch2@jh.edu

 

Seth Blackshaw, Ph.D., Faculty Director
MRB Room 339
Email: sethblackshaw@gmail.com

 

Location and hours of operation

Hours Location

Hours Open: Monday - Friday 8:30am-5:00pm

Hours Fully Staffed: Monday - Friday 8:30am-5:00pm

733 N Broadway
MRB 360
Baltimore, MD 21205

Links and Resources

1. Transcriptomics and Deep Sequencing Core (hopkinsmedicine.org)

Contacts

Name Role Phone Email Location
Linda D. Orzolek, MS
Director
 
410-502-6658
 
ldorsch2@jhmi.edu
 
MRB Room 363
 
Seth Blackshaw, PhD
Faculty Director
 

 
sethblackshaw@gmail.com
 
MRB Room 335
 
Jasmeet Sethi, PhD
Sr. Research Specialist
 
443-287-8852
 
jsethi1@jhmi.edu
 
MRB Room 355
 
Conover Talbot Jr., BS
Bioinformatician
 
410-614-0443
 
cct@jhmi.edu
 
MRB Room 353
 

Price list


Search available services: View: by category alphabetically
Data Analysis (3)
Microarrays (26)
Nanostring (3)
Nucleic Acid Extraction (7)
PacBio (4)
Quality Control (9)
RT PCR (1)
Scanning (3)
Sequencing (36)
Shearing (4)
Single Cell Sequencing (9)

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