Our aim is to provide cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. The Core has been in continuous operation since 2002 and has supported research projects for more than 467 principle investigators, 1097 individual researchers, and processed more than 36,000 samples (as tallied in April, 2016). We offer sample preparation and analysis using Next Generation & Third Generation sequencing technologies, Microarrays, Nanostring nCounter, and qPCR. We also offer high throughput DNA, Chromatin and RNA shearing using Covaris E220 system. Our lab is equiped with the PacBio RSII for third generation, long read, single-molecule real-time (SMRT) sequencing, as well as the Illumina NextSeq 500 and MiSeq next-generation sequencer for short reads. We work closely with the GRCF to enable our clients access to the Illumina HiSeq 2500/3000 for deeper sequencing requirements. The PacBio SMRT sequencing technology is great for genome assembly, Iso-Seq for novel transcript isoform identification, full length 16s rRNA gene sequencing for metagenomics, repeat sequence and GC-rich region targeted resequencing, as well as for bacterial DNA methylation study. The Illumina short read sequencing technology, with its high throughput, is great for whole genome resequencing, whole exome sequencing, RNAseq, ChIPseq, 16s rRNA gene or whole genome metagenomic sequencing, and targeted DNA resequencing. We run the Affymetrix microarray system, while offering preparation and support for Agilent arrays as well. In addition, we offer the Nanostring nCounter system for RNA expression, FFPE analysis, CNV, and miRNA detection. We ensure a full service experience for our clients, starting with project design, all the way through publication. We encourage our clients to meet with us before beginning any projects to ensure the most accurate and efficient results. From nucleic acid extraction through analysis, we are your one stop shop.
Seth Blackshaw, Ph.D. Director
BRB Room 335
Haiping Hao, Ph.D., Associate Director
BRB Room 359
Office Phone: (443) 287-9056
Lab Phone: (410) 502-2008
Hours Open: Monday - Friday 8:30am-7:00pm
Hours Fully Staffed: Monday - Friday 8:30am-5:00pm
733 N Broadway
|► Data Analysis (2)|
Monthly subscription to Ingenuity Pathway Analysis. This service requires user to register an account with Ingenuity. It is billed monthly, regardless of time used.
|► Microarrays (21)|
|3' Expression (10+)||Inquire|
|3' Expression, NuGEN||Inquire|
|3' Expression, NuGEN (10+)||Inquire|
|Exon array (10+)||Inquire|
|Gene 1.0/2.0 ST array||Inquire|
|Gene 1.0/2.0 ST array (10+)||Inquire|
|miRNA array (10+)||Inquire|
|Promoter array (10+)||Inquire|
|Transcriptome array (10+)||Inquire|
|► Nanostring (3)|
|Gene Expression Assay||Inquire|
|► Nucleic Acid Extraction (4)|
|DNA extraction (10+)||Inquire|
|RNA extraction (10+)||Inquire|
|► PacBio (4)|
|Pacbio sequencing 8||Inquire|
|► Quality Control (4)|
|QC only (10+)||Inquire|
|RUSH QC only||Inquire|
|RUSH QC only (10+)||Inquire|
|► RT PCR (1)|
|Real time PCR||Inquire|
|► Scanning (3)|
|Hyb and Scan||Inquire|
|Hyb and Scan (10+)||Inquire|
|► Sequencing (29)|
|16S rRNA seq||
Metagenomic sequencing targeting 16s rRNA variable region 3 and 4. Requires 50 samples minimum. Fewer samples will resulting in higher prices. Sequenced on MiSeq for 2X300 bp and targeting 50,000 paired reads per sample.
|ChIPseq library preparation||
ChIPseq library preparation using Nugen Ultra Low Input kit. Requires 1-10ng of ChIP DNA as input.
|DNAseq library preparation||Inquire|
|DNAseq library preparation (10+)||Inquire|
|NextSeq 500 High 150||
NextSeq 500 sequencing using High Output 150 cycle reagents for single 150bp reads, or paired 75bp reads. Yield up to 400 million single/paired reads.
|NextSeq 500 High 300||
NextSeq 500 sequencing using High Output 300 cycle reagent kit for paired 150bp reads. Yield up to 400 million paired reads.
|NextSeq 500 High 75||
NextSeq 500 sequencing using High Output 75 cycle reagent kit for single 75bp reads. Yield up to 400 million reads.
|NextSeq 500 Mid 1X150||
NextSeq 500 sequencing using Mid Output 150 cycle kit for single 150bp reads.
|NextSeq 500 Mid 2X150||
NextSeq 500 sequencing using Mid Output 300 cycle reagent kit for paired 150bp reads. Yield upto 130 Million paired reads.
|small RNAseq library prep||Inquire|
|stranded mRNAseq library prep||
mRNA library prep for standard or low input samples
|stranded mRNAseq library prep (10+)||
mRNA library prep for standard and low input amounts when 10 or more samples are submitted as one project
|stranded Total RNAseq library prep||
Total RNA library prep for standard and low input amounts
|Stranded Total RNAseq library prep (10+)||
Total RNA library prep for standard and low input amounts when 10 or more samples are submitted as one project
|Whole Exome Sequencing (Human)||Inquire|
|Whole Exome Sequencing (Human, 10+)||Inquire|
|► Shearing (4)|
|Covaris shearing (10+)||Inquire|
|gTube Shearing (10+)||Inquire|