The Genetic Resources Core Facility (GRCF) is a Johns Hopkins service center providing research expertise, products, and services for the study of the human genome. We strive to streamline services such as sample collection, cell line establishment, cell line authentication, cryogenic storage, DNA isolation, oligo and gene synthesis, methylation testing, both Sanger and next-gen sequencing as well as genotyping from handfuls to millions of variants. The GRCF can also help with project design and custom assay development for DNA based studies.
We are a part of Johns Hopkins Genomics and function as three units: the Core Store, the Cell Center & Biorepository and DNA Services. This site is for ordering some of the services offered by the DNA Services unit. Other services, including next-gen sequencing, may be ordered on our Genesifter server. The Cell Center & Biorepository and the Core Store maintain separate ordering sites.
Roxann Ashworth - Co-Director (Blalock)
Email: rashwor2@jhmi.edu
Phone: 410-614-0702
Location: Blalock 1004
Laura Kasch-Semenza - Co-Director (LHP)
Email: lkasch@jhmi.edu
Phone: 410-614-3830
Location: 2760 Lighthouse Point East, Suite 201, Baltimore, MD 21224
General questions should be addressed to the DNA services contact point: customorders@jhmi.edu; 410-955-2836.
General Lab contact - Blalock Location
Email: customorders@jhmi.edu
Phone: 410-955-2836
Oligo order questions - Blalock Location
Email: customorders@jhmi.edu
Phone: 410-955-2836
General Lab contact - LHP Location
Email: faf@jhmi.edu
Phone: 443-287-7948
Location: 600 N. Wolfe St. Blalock 1004. Baltimore, MD 21287
Hours: Monday - Friday : 7am - 4:30pm
Location: 2760 Lighthouse Point East, Suite 200. Baltimore, MD 21224
Hours: Monday - Friday: 8:30am - 4:30pm
Samples for the LHP location may be dropped off at the Blalock location or one of our remote drop box locations.
Remote drop off locations:
Name | Role | Phone | Location | |
---|---|---|---|---|
General Lab contact |
Blalock location
|
410-955-2836
|
customorders@jhmi.edu
|
Blalock 1004
|
General Lab contact |
LHP location
|
443-287-7948
|
faf@jhmi.edu
|
Lighthouse Point
|
Oligo order questions |
Blalock location
|
410-955-2836
|
customorders@jhmi.edu
|
Blalock 1004
|
Roxann Ashworth |
Co-Director
|
410-614-0702
|
rashwor2@jhmi.edu
|
Blalock 1004
|
Laura Kasch-Semenza |
Co-Director
|
410-614-3830
|
lkasch@jhmi.edu
|
2760 Lighthouse Point East, Suite 201, Baltimore, MD 21224
|
Breon Beech |
Lab Manager
|
410-955-2836
|
bbeech@jhmi.edu
|
Blalock 1004
|
Service list |
► DNA Isolation (8) | |||
Name | Description | Price | |
---|---|---|---|
Core punches: 1 @ <1.2mm diameter |
Covaris truXTRAC Covaris truXTRAC or Qiagen |
Inquire | |
DNA Isolation FFPE block 5-10mg | Inquire | ||
DNA Isolation FFPE Slides - 1-2 slides; 4-10um |
(~200mm2 for a 5µm section), Covaris truXTRAC or Qiagen |
Inquire | |
DNA Isolation FFPE Slides - 3-4 slides; 4-10um |
(~200mm2 for a 5µm section), Covaris truXTRAC or Qiagen |
Inquire | |
Scrolls/Curls 1 @ 16-25µM, <10 mm length |
Covaris truXTRAC Covaris truXTRAC or Qiagen |
Inquire | |
Scrolls/Curls 1-2 @ 7-15µM <10 mm length |
Covaris truXTRAC Covaris truXTRAC or Qiagen |
Inquire | |
Scrolls/Curls 2 @ 16-25µM <10 mm length |
2 preps, Covaris truXTRAC Covaris truXTRAC or Qiagen |
Inquire | |
Scrolls/Curls 2-4 @ 7-15µM <10 mm length |
2 preps, Covaris truXTRAC Covaris truXTRAC or Qiagen |
Inquire | |
► Digital PCR (1) | |||
Name | Description | Price | |
Digital PCR - Constellation | Inquire | ||
► Genotyping - Illumina - GWAS (42) | |||
Name | Description | Price | |
CytoSNP-850 - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
CytoSNP-850K - Full Service (94-2,000 Samples) |
Full service includes:
|
Inquire | |
CytoSNP-850K - No Pretesting (93 or fewer Samples) |
Full service includes:
|
Inquire | |
CytoSNP-850K - No pretesting (94-2,000 Samples) |
This service includes:
|
Inquire | |
Global Diversity Array - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
Global Diversity Array - Full Service (5000+ samples) |
Full service includes:
|
Inquire | |
Global Diversity Array - Full Service (94-5000 samples) |
Full service includes:
|
Inquire | |
Global Diversity Array - No Pretesting (93 samples) |
Full service without Pretesting includes:
|
Inquire | |
Global Diversity Array - No Pretesting (94-5000) |
Full service without Pretesting includes:
|
Inquire | |
Global Screening Array - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
Global Screening Array - Full service (94-2000 samples) |
Full service includes:
|
Inquire | |
Global Screening Array - No Pretesting (94-2000) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Core Array - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Core Array+exome - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
Mega EUR/EAS/SAS subset - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Mega Global - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Mega Global - Alternative Service Level |
For customers who do not want one of the standard service options. Pricing to be determined by services selected. |
Inquire | |
Mega Global - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
Omni 2.5M - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 2.5M - Alternative Service Level |
For customers who do not want one of the standard service options. Pricing to be determined by services selected. |
Inquire | |
Omni 2.5M - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
Omni 2.5M - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 2.5M+exome - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 2.5M+exome - Alternative Service Level |
For customers who do not want one of the standard service options. Pricing to be determined by services selected. |
Inquire | |
Omni 2.5M+exome - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
Omni 2.5M+exome - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 5M - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 5M - Alternative Service Level |
For customers who do not want one of the standard service options. Pricing to be determined by services selected. |
Inquire | |
Omni 5M - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
Omni 5M - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 5M+exome - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 5M+exome - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
Omni 5M+exome - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
Omni 5M+exome - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
OmniExpress - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
OmniExpress - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
OmniExpress - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
OmniExpress - Alternative Service Level |
For customers who do not want one of the full service options. Pricing to be determined by services selected. |
Inquire | |
OmniExpress+exome - No Pretesting (93 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
OmniExpress+exome - Full Service (94-2000 samples) |
Full service includes:
|
Inquire | |
OmniExpress+exome - No Pretesting (94-2000 samples) |
This service level includes:
Samples will be run as submitted, with no pretesting. |
Inquire | |
OmniExpress+exome - Alternative Service Level |
For customers who do not want one of the standard service options. Pricing to be determined by services selected. |
Inquire | |
► Genotyping: Non-Human (1) | |||
Name | Description | Price | |
Mouse GIGAmuga - Full Service (84-2000 samples) | Inquire | ||
► Genotyping: Specialized Content Options (8) | |||
Name | Description | Price | |
Exome array - Full Service (93 samples) |
This service level includes:
|
Inquire | |
Exome array - Full Service (94-2000 samples) |
This service level includes:
|
Inquire | |
Exome array - No Controls & Redos (93 samples) |
This service level includes:
We will not supply any controls nor will we redo any samples that fail. Each sample will be run once in the lab. |
Inquire | |
Exome array - No Controls & Redos (94-2000 samples) |
This service level includes:
We will not supply any controls nor will we redo any samples that fail. Each sample will be run once in the lab. |
Inquire | |
Exome array - No controls/ Raw data only (93 samples) |
This service level includes:
We will not run a technical filter on your data, so you will need to clean and remove all failed assays from the provided data set. To visualize and clean the data you will need access to a copy of GenomeStudio from Illumina or similar software from a 3rd party vendor that uses .idat files as input. Our data release will include the standard .egt file from Illumina, but it will not have been optimized for your specific data.
You should expect an FTE data analyst to spend at least two weeks cleaning the data after you receive it from us. |
Inquire | |
Exome array - No controls/ Raw data only (94-2000 samples) |
This service level includes:
We will not run a technical filter on your data, so you will need to clean and remove all failed assays from the provided data set. To visualize and clean the data you will need access to a copy of GenomeStudio from Illumina or similar software from a 3rd party vendor that uses .idat files as input. Our data release will include the standard .egt file from Illumina, but it will not have been optimized for your specific data.
You should expect an FTE data analyst to spend at least two weeks cleaning the data after you receive it from us. |
Inquire | |
Exome array - Raw Data only + PLINK release (93 samples) |
This service level includes:
We will not run a technical filter on your data, so you will need to clean and remove all failed assays from the provided data set. To visualize and clean the data you will need access to a copy of GenomeStudio from Illumina or similar software from a 3rd party vendor that uses .idat files as input. Our data release will include the standard .egt file from Illumina, but it will not have been optimized for your specific data.
You should expect an FTE data analyst to spend at least two weeks cleaning the data after you receive it from us. |
Inquire | |
Exome array - Raw Data only + PLINK release (94-2000 samples) |
This service level includes:
We will not run a technical filter on your data, so you will need to clean and remove all failed assays from the provided data set. To visualize and clean the data you will need access to a copy of GenomeStudio from Illumina or similar software from a 3rd party vendor that uses .idat files as input. Our data release will include the standard .egt file from Illumina, but it will not have been optimized for your specific data. You should expect an FTE data analyst to spend at least two weeks cleaning the data after you receive it from us. |
Inquire | |
► Methylation Array (1) | |||
Name | Description | Price | |
FFPE restore |
This special process repairs highly fragmented DNA (such as from FFPE or CF DNA) so that it is usuable on any Illumina array. |
Inquire | |
► Pipette Services (3) | |||
Name | Description | Price | |
8 or fewer Channel Multi-channel Pipette Calibration |
For 8 channel or less pipettes. |
Inquire | |
9 or more Channel Multi-Channel Calibration |
For pipettes with 9 or more channels. |
Inquire | |
Single Channel Pipette Calibration | Inquire | ||
► Sequencing (2) | |||
Name | Description | Price | |
Oxford Nanopore Library prep |
Service for ONT library prep |
Inquire | |
Oxford Nanopore Sequencing |
Service for ONT sequencing. |
Inquire | |
► Taqman Open Array Gene Expression chips (7) | |||
Name | Description | Price | |
Human Barcode | Inquire | ||
Taqman OpenArray Custom Chip processing Costs - 16 assays |
The processing costs and chip costs are billed separately. This charge is for the processing costs, which vary depending on the type of chip and number of chips processed. Price shown is for 10 chips, price will be adjusted based on actual number of chips run. |
Inquire | |
Taqman OpenArray Custom Chip processing Costs - 56 or more assays |
The processing costs and chip costs are billed separately. This charge is for the processing costs, which vary depending on the type of chip and number of chips processed. Price shown is for 20 chips, price will be adjusted based on actual number of chips run. |
Inquire | |
Taqman OpenArray Custom Genotyping Chips - 12 SNPs |
Minimum order 10 chips, 144 samples per chip |
Inquire | |
Taqman OpenArray Custom Genotyping Chips - 180 SNPs |
Minimum order 60 chips, 16 samples/chip |
Inquire | |
Taqman OpenArray Genotyping Chips - 26 SNPs |
Minimum order 10 chips, 96 samples/chip. |
Inquire | |
Mouse Inflammation Chip (1 Chip) | Inquire |