Bayview Immunomics Core (BIC)

Overview of Services

 

The Bayview Immunomics Core (BIC) was initially established in 2008 as a flow cytometry core to meet the biomedical research needs of Johns Hopkins Investigators located on the Bayview Campus. We have since expanded to include immunoassays and renamed the core the BIC.  The flow cytometry service center is uniquely embedded among research groups interested in problems related to immune based human diseases and relevant animal models and promotes an interactive and collaborative approach.  We are presently equipped with a Cytek Aurora 5-laser spectral flow cytometer with a 40-parameter capability, a special order FACSAria cell sorter (BD Biosciences) with 18-fluorescence parameter capability, and analysis workstations for off-line data analysis. The instruments are maintained using the highest standards of quality control and are available for analysis to certified and trained users. Cell sorting is performed by flow core staff only. Our staff offers free consultation services.

 

We also have on site a Meso Scale Diagnostics multi-analyte system capable of sensitive (subnanogram) multiplex based detection of soluble mediators in fluid sources. Meso Scale developed an electrochemiluminescence-based detection system that offers several advantages in comparison to standard ELISAs including high sensitivity, a broad dynamic range, low background and increased reproducibility.  This instrument employs standard or custom plates, and assays can be run and read by core or investigator's lab staff. Access requires an initial consult.

 

 

Leadership

Franco D'Alessio, MD - Director
Email: fdaless2@jhmi.edu (preferred way to be contacted)
Phone: 410-245-3047

Hong Wang, MD, PhD - Administrator
Email: hwang154@jhmi.edu (preferred way to be contacted)
Phone: 410-550-1029

 

Location and hours of operation

Hours:

Monday - Friday 

9:00am - 5:00pm 

After hour use of the facility available to trained users, or upon request
for sorting. 

 

Location:

Mason Lord Building, Center Tower
Suite 5000, Rm 548
5200 Eastern Av
Baltimore, MD 21224 

 

Links and Resources

  1. Bayview Flow Cytometry Website
  2. http://oacapps.med.jhmi.edu/bb/soloski.html
  3. http://www.bdbiosciences.com/en-us/support/training/e-learning-courses
  4. http://www.lifetechnologies.com/us/en/home/support/tutorials.html  
  5. http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer
  6. https://cytekbio.com/

Contacts

Name Role Phone Email Location
Franco D'Alessio
Director
 
410-245-3047
 
fdaless2@jhmi.edu
 
JHAAC 4A 62
 
Hong Wang
Administrator
 
410-550-1029
 
hwang154@jhmi.edu
 
MFL5, Suite 5200
 
Marta Escarra Senmarti
FACS Operator
 

 
mescarr1@jhmi.edu
 
MFL6
 
Daniela Flores
MSD Operator
 
410-550-1863
 
mvilleg5@jhu.edu
 
MFL6
 
David Hines
Emergency Contact
 

 
dhines6@jhmi.edu
 
MFL5, Suite 5300