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Bayview Immunomics Core (BIC)

Overview of Services

 

The Bayview Immunomics Core (BIC) was initially established in 2008 as a flow cytometry core to meet the biomedical research needs of Johns Hopkins Investigators located on the Bayview Campus. We have since expanded to include immunoassays and renamed the core the BIC. The BIC is directed by Erika Darrah, Ph.D. The flow cytometry service center is managed by Ningchun Xu, PhD, SCYM (ASCP) who brings the highest standard of flow cytometry experience to the modern biomedical sciences. The core is uniquely embedded among research groups interested in problems related to immune based human diseases and relevant animal models and promotes an interactive and collaborative approach. We have an extensive research base and considerable expertise in flow based approaches to address neuroscience related research. The flow cytometry instrumentation has the capability of performing simple and complex multiparametric (up to 18 fluorochromes) analysis of cell subpopulations and high speed sorting. We are presently equipped with a special order FACSAria cell sorter (BD Biosciences) with 18-fluorescence parameter capability and analysis workstation for off-line data analysis. The FACSAria is maintained using the highest standards of quality control and are available for analysis to certified and trained users. Cell sorting is performed by flow core staff only. Our staff offers free consultation services.

 

We also have on site a Meso Scale system capable of sensitive (subnanogram) multiplex based detection of soluble mediators in fuid sources. Meso Scale developed an electrochemiluminescence-based detection system that offers several advantages in comparison to standard ELISAs including high sensitivity, a broad dynamic range, low background and increased reproducibility.  This instrument employs standard or custom plates, and assays can be run and read by core or investigator's lab staff. Access requires an initial consult.

 

Leadership

Erika Darrah, PhD - Director
Email: edarrah1@jhmi.edu (preferred way to be contacted)
Phone: 410-550-1029

Ningchun Xu, Ph.D. Flow Core Manager
Email: nxu9@jhmi.edu (preferred way to be contacted)
Phone: 410-550-8291
Fax: 410-550-1896

 

Location and hours of operation

Hours:

Monday - Friday 

9:00am - 5:00pm 

After hour use of the facility available to trained users, or upon request
for sorting and users that need assistance. Contact Lab Manager. 

 

Location:

Mason Lord Building, Center Tower
Suite 5000, Rm 548
5200 Eastern Av
Baltimore, MD 21224 

 

Links and Resources

  1. Bayview Flow Cytometry Website
  2. http://oacapps.med.jhmi.edu/bb/soloski.html
  3. http://www.bdbiosciences.com/en-us/support/training/e-learning-courses
  4. http://www.lifetechnologies.com/us/en/home/support/tutorials.html  
  5. http://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer

Contacts

Name Role Phone Email Location
Erika Darrah, PhD
Director
 
410-550-7340
 
edarrah1@jhmi.edu
 
Mason Lord Building Center Tower, Room 5300
 
Ningchun Xu, PhD
Lab Manager
 
410-550-8291
 
nxu9@jhmi.edu
 
MLB CT, Rm 548
 
Marcia Villegas de Flores
Research Specialist
 
410-550-1863
 
mvilleg5@jh.edu
 
MLB CT, Rm 684