High Throughput Screening (ChemCORE)

High Throughput Screening Services

As a screening center for the NIH Molecular Libraries Probe Centers Network (MLPCN), Johns Hopkins ChemCORE is a well established high throughput screening facility which has carried out 100+ projects for drug discovery community in the past 15 years.

ChemCORE provides a variety of services and expertise with automation and detection capabilities to make drug discovery process streamlined and fast:

• Screening with in house large libraries:
  - NIH full collection (300,000 compounds) 
  - ChemBridge Diverse Set (50,000), ChemBridge CNS Set (50,000 compounds) 
  - Newly arrived ChemDiv Diverse Set (100,000) and its repicked cost-saving set (10,000 compounds)
  - Newly replenished and expanded Johns Hopkins Drug Library - JHDLv3 (4,500 drugs)
• Screening with FDA approved drug collection for possible repurposing/repositioning
• Pilot or proof of concept screening with special smaller libraries: MicroSource Spectrum (2,000) and Sigma LOPAC(1,280)
• Assay development and implementation: cell based and protein based assays, on 96/384/1536 well format
• Automating many processes other than compound screening: RNA-Seq, MODLI-TOF, large scale sample preparation and reformatting
• Support letter for grant application
• Equipment user training

Advanced Instruments

• BMG CLARIOstar multimode microplate reader: luminescence, florescence, polarization.
• Molecular Devices SpectraMax i3X multimode plate reader, with online reagent dispensing
• BD Pathway™ 855 Bioimager: high speed automated cell imaging
• FDSS6000 and FDSS7000 System: florescence/luminescence kinetic reading
• Epic ™ System: label-free detection on 384-well format
• Liquid handling automation, more than 5 workstations for wide range of volume for 96/384/1536-well plates
• Echo 650 acoustic dispenser, non-contact, as low as 2.5nL

BIG PICTURE SCIENCE

Jun Liu on Looking to Nature for New Medications

Leadership

Location and hours of operation

Contacts