High Throughput Screening (ChemCORE)

High Throughput Screening Services

As a screening center for the NIH Molecular Libraries Probe Centers Network (MLPCN), Johns Hopkins ChemCORE is a well established high throughput screening facility which has carried out 100+ projects for drug discovery community.

ChemCORE provides a variety of services and expertise with automation and detection capabilities to make drug discovery process streamlined and fast:

• Screening with in house large libraries: NIH collection (300,000 compounds), ChemBridge Diverse Set (50,000), CNS Set (50,000)
• Screening with FDA approved drug collection for possible repurposing/repositioning
• Pilot or proof of concept screening with special smaller libraries: MicroSource Spectrum (2,000) and Sigma LOPAC(1,280)
• High throughput ion channel test: special expertise with membrane protein targets
• Assay development and implementation: cell based and protein based assays, on 96/384/1536 well format
• Automating many processes other than compound screening: RNA-Seq, MODLI-TOF, large scale sample preparation and reformatting
• Genomic Resources: collections of Ultimate ORFs, human/mouse shRNA clones, and YKO strains (order online: http://hitcores.bs.jhmi.edu)
• Support letter for grant application
• Equipment user training

Advanced Instruments

• Tecan Safire²™ multimode microplate reader: luminescence, florescence, polarization.
• Analyst GT plate reader: high throughput
• BD Pathway™ 855 Bioimager: high speed automated cell imaging
• FDSS6000 and FDSS7000 System: florescence/luminescence kinetic reading
• Ion Channel Reader 8000: atomic absorption
• Ionworks Quattro, 2 units, high throughput automatic patch clamping.
• QPatch, high resolution automatic patch clamping.
• Epic ™ System: label-free detection on 384-well format
• Liquid handling automation, more than 5 workstations for wide range of volume for 96/384/1536-well plates

Leadership

Location and hours of operation

Links and Resources

1. ChemCORE Facility
2. ChemCORE Services
3. ChemCORE FAQ

Contacts