High Throughput Screening (ChemCORE)

High Throughput Screening Services

As a screening center for the NIH Molecular Libraries Probe Centers Network (MLPCN), Johns Hopkins ChemCORE is a well established high throughput screening facility which has carried out 100+ projects for drug discovery community in the past 15 years.

ChemCORE provides a variety of services and expertise with automation and detection capabilities to make drug discovery process streamlined and fast:

  • Screening with in house large libraries:
    - NIH full collection (300,000 compounds) 
    - ChemBridge Diverse Set (50,000), ChemBridge CNS Set (50,000 compounds) 
    - Newly arrived ChemDiv Diverse Set (100,000) and its repicked cost-saving set (10,000 compounds)
  • Screening with FDA approved drug collection for possible repurposing/repositioning
  • Pilot or proof of concept screening with special smaller libraries: MicroSource Spectrum (2,000) and Sigma LOPAC(1,280)
  • Assay development and implementation: cell based and protein based assays, on 96/384/1536 well format
  • Automating many processes other than compound screening: RNA-Seq, MODLI-TOF, large scale sample preparation and reformatting
  • Support letter for grant application
  • Equipment user training

Advanced Instruments

  • Tecan Safire²™ multimode microplate reader: luminescence, florescence, polarization.
  • Molecular Devices SpectraMax i3X multimode plate reader, with online reagent dispensing
  • BD Pathway™ 855 Bioimager: high speed automated cell imaging
  • FDSS6000 and FDSS7000 System: florescence/luminescence kinetic reading
  • Epic ™ System: label-free detection on 384-well format
  • Liquid handling automation, more than 5 workstations for wide range of volume for 96/384/1536-well plates

Leadership

Jun Liu, PI

     

    

Alan Long, Manager

Professor

(410)955-4619

joliu@jhu.edu

 

 

Sr. Research Engineer

(410)502-3069

slong15@jhmi.edu

Location and hours of operation

Hours Location

Monday - Friday  

9am - 5pm

Miller Research Building 346

733 North Broadway, Baltimore, Maryland 21205

Links and Resources

  1. ChemCORE Facility

Contacts

Name Role Phone Email Location
Jun Liu
PI
 
(410)955-4619
 
joliu@jhu.edu
 
Hunterian 516
 
Alan Long
Lab Manager
 
(410)502-3069
 
slong15@jhmi.edu
 
MRB 349
 
Hongyu Xu
Sr. Research Specialist
 
(443)287-4411
 
hxu41@jhmi.edu
 
MRB 347