Genetic Resources Core Facility Biorepository & Cell Center

20lb Dry Ice

60lb Dry Ice

6lb Dry Ice

Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Alliquoting of Whole Blood- per sample.

Yearly Storage per vial

July 1- June 30 coverage 

Total charge initiated in July for investigators with less than 500 samples.

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Shipment of biological specimens not stored within the biorepository.

Cost per card

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

PicoGreen as assessed in FAF core.

5 x 10^6 cells/vial cryopreserved

Less than 5/month expected

Less than 10 / month

Less than 20/month

Individual consultation/lab or biosafety cabinet cleaning available by appointment. We are available to help with various cell culture issues and/or problems. Fees are based on hourly rates.

Growth of culture for 14 days with visual inspection of contamination

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

Cell quantitation, loading & QC imaging using Fluidigms C1. Includes: stPreAmp IFC, DNA IFC (size specific), cell counts and size measurements, tube control, images of wells

DNA Isolation by FAF

Dry Ice_per 1 pound

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner.Cryopreservation per vial and banked at -80C.Database entry for all aliquots.

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.Cryopreservation of per vial and banked at -80C.Database entry for all aliquotes.

FedEx Charges

Aliquoting of cells, sera/plasma or other biological agents (per aliquot)

Establish primary cells from skin biopsy.  Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Q/C FAIL Attempt for Transformation after improper sampling techniques by investigator observed and recorded. (Images availible upon request)

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a 1 liter roller bottle

Growth of an established adherent cell line in a T150/T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Q/C FAIL Attempt for expansion after improper sampling techniques by investigator observed and recorded. (Images available upon request)

Growth of established cell lines in multi-well tissue culture dishes.

Preparation of cell pellet at 100,000,000 cells

Growth of an established suspension cell line in a 1 liter roller bottle

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell line in a T300 flask

Growth of established suspension cell lines in a T75 flask

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Preparation of cell pellet (100,000,000 cells)

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (E), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Specimen contaminated upon receipt

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Generation of labels based on File

Package UN3373 Large 60lb Box 24"x16"x16"

LN2 as supplied for the cryocart or other use, charged by the liter

Package UN3373 Medium 20lb Box 14"x14"14"

Cell line deposit: 5 lines, no distribution

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Mycoplasm Test after completion

Cryopreservation of cells (already expanded) for storage in LN2/ vial

Quarterly storage per vial

For Investigators with >500 samples

Isolation of RNA from cultured cells by FAF

Package UN3373 Small 6lb Box 11"x9"x7"

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Disposal with Certificate of Discard

Sample Retrieval. Removing 1 vial from the liquid nitrogen freezer or minus 80 freezer.

Administrative form fee for transfer of sample aliquots from Originating Principal Investigator to a recipient PI (JHMI or outside).

Supplies needed for modified protocol

WorldCourier Charges

Cell quantitation, loading & QC imaging using Fluidigms C1. E Includes: stPreAmp IFC, mRNA IFC (size specific), cell counts and size measurements, tube control, images of wells

Cell quantitation, loading & QC imaging using Fluidigms C1. Includes: stPreAmp IFC (size specific), cell counts and size measurements, tube control, images of wells

LN2 as supplied for the cryocart or other use, charged by the liter

Lymphocyte isolation and cryopreservation. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Database entry for all aliquots.

Fresh WB Transformation and T25 flask. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells. Transformation of fresh lymphocytes with Epstein-Barr virus.Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6cells. Database entry for all aliquotes. Allow 4 weeks for completion.

Fresh WB Transformation and hold. Lymphocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6 cells. Transformation of fresh lymphocytes with Epstein-Barr virus. Cryopreservation of 4 vials (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow4-5 weeks for completion.

Fresh WB Transformation with bulk growth. Lymphocyte separation. Cell count.Viability determination. Cryopreservation of whole blood (1ml). Cryopresarvation of 1-4 vials (@5x10E6 each) of non-transformed lymphocytes @5x10E6cells.Transformation of fresh lymphocytes with Epstein-Barr virus. Bulk growth (1x10E8) of transformed cells. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 6-8 weeks for completion.

Isolation and cryopreservation of plasma. Plasma is separated by centrifugation, recovered in a sterile manner.Cryopreservation per vial and banked at -80C.Database entry for all aliquots.

Isolation and cryopreservation of sera. Sera is separated by centrifugation, recovered in a sterile manner.Cryopreservation of per vial and banked at -80C.Database entry for all aliquotes.

Thaw and transformation of lymphocytes w/EBV. Culture to 5x106 cells/ml, suitable for cryopreservation of 2 vial transformed lymphocytes. Allow 4 weeks for completion.

All services listed in (G), plus 2 additional cryopreserved vials of transformed lymphocytes (at 5x106 cells/ml) (Total 4).  Allow 4-5 weeks for completion.

All service in (E), plus culture to 1x108 cells/ml suitable for DNA analysis (cell pellet).  Allow 6-8 weeks for completion.

Culture suitable for T25 pick up and cryopreservation of 1 replacement vial (@ 5x10^6 cells/ml).  Allow 3-5 for completion.

Culture to 1x108 cells/ml suitable for DNA analysis (cell pellet) and cryopreservation of 1 replacement vial (@5x10^6 cells/ml).  Allow 2-3 weeks for completion.

Alliquoting of Whole Blood- per sample.

Monocyte isolation and cryopreservation. Lymphocyte & Monocyte separation. Cell count. Viability determination. Cryopreservation of whole blood (1ml). Cryopreservation of 1-4 vials (@5x10E6 each) of non-transformed monocytes @5x10E6cells. Database entry for all aliquots

Preparation of cell pellet at 100,000,000 cells

5 x 10^6 cells/vial cryopreserved

Growth of culture for 14 days with visual inspection of contamination

Aliquoting of cells, sera/plasma or other biological agents (per aliquot)

Establish primary cells from skin biopsy.  Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Cell culture expansion of established cell line, cryopreservation of replacement vial @5e6 cells/ml and Cell Pellet Preparation. Culture to 10e8 cells suitable for DNA analysis.

Growth of an established adherent cell line in a 1 liter roller bottle

Growth of an established adherent cell line in a T150/T175 flask

Growth of an established adherent cell line in a T25 flask

Growth of an established adherent cell line in a T75 flask

Growth of established cell lines in multi-well tissue culture dishes.

Growth of an established suspension cell line in a 1 liter roller bottle

Growth of established suspension cell lines in T25 flask

Growth of established suspension cell line in a T300 flask

Growth of established suspension cell lines in a T75 flask

Subsequent bulk growth. Bulk growth (1x10E8 cells) of a transformed established cell line. Cryopreservation of cell pellet or 20 vials of transformed lymphoblasts. Allow approximately 2-3 weeks for completion.

Subsequent growth. T25 flask (5x10E6 cells) of a transformed cell line. Allow approximately 3-5 days for completion. (Replacement of initial vial)

Subsequent transformation and T25 flask. Transformation of frozen lymphocytes with Epstein-Barr virus. Cryopreservation of 2 vial of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4 weeks for completion.

Subsequent transformation with bulk growth. Transformation of cryopreserved lymphocytes with Epstein-Barr virus.Bulk growth (1x10E8) of transformed. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquotes. Allow approximately 6-8 weeks for completion.

Subsequent transformation and hold. Transformation of frozen lymphocyteswith Epstein-Barr virus. Cryopreservation of 4 vials of (transformed) lymphoblasts @5x10E6 cells. Database entry for all aliquots. Allow 4-5weeks for completion.

Preparation of cell pellet (100,000,000 cells)

Prepare frozen stocks @ 5x10E6 cells each of established cell lines.

Cryopreservation of cells (already expanded) for storage in LN2/ vial

Less than 5/month expected

Less than 10 / month

Less than 20/month

Cell line deposit: 5 lines, no distribution

Q/C FAIL Attempt for expansion after improper sampling techniques by investigator observed and recorded. (Images available upon request)

Supplies needed for modified protocol

Q/C FAIL Attempt for Transformation after improper sampling techniques by investigator observed and recorded. (Images availible upon request)

Specimen contaminated upon receipt

Defined dates and times for patient sample processing: including technician monitoring of critical processing time points, refrigerated centrifuge, and aliquoting on ice with snap freezing.

Individual consultation/lab or biosafety cabinet cleaning available by appointment. We are available to help with various cell culture issues and/or problems. Fees are based on hourly rates.

Cell quantitation, loading & QC imaging using Fluidigms C1. Includes: stPreAmp IFC, DNA IFC (size specific), cell counts and size measurements, tube control, images of wells

Cell quantitation, loading & QC imaging using Fluidigms C1. E Includes: stPreAmp IFC, mRNA IFC (size specific), cell counts and size measurements, tube control, images of wells

Cell quantitation, loading & QC imaging using Fluidigms C1. Includes: stPreAmp IFC (size specific), cell counts and size measurements, tube control, images of wells

Isolation of RNA from cultured cells by FAF

Mycoplasm Test after completion

DNA Isolation by FAF

PicoGreen as assessed in FAF core.

Purchase of a 9 x 9 Cryo Storage Box, suitable for long term storage.

Purchase of Envelope + Dry Ice for Customer Biospecimen Transport

Administrative form fee for transfer of sample aliquots from Originating Principal Investigator to a recipient PI (JHMI or outside).

Generation of labels based on File

User and PI must sign CryoCart/Cryogenic Liquids User Responsibility Agreement prior to use of CryoCart.

Package UN3373 Small 6lb Box 11"x9"x7"

Package UN3373 Medium 20lb Box 14"x14"14"

Package UN3373 Large 60lb Box 24"x16"x16"

6lb Dry Ice

20lb Dry Ice

60lb Dry Ice

Dry Ice_per 1 pound

FedEx Charges

Shipment of biological specimens not stored within the biorepository.

WorldCourier Charges

Yearly Storage per vial

July 1- June 30 coverage 

Total charge initiated in July for investigators with less than 500 samples.

Quarterly storage per vial

For Investigators with >500 samples

Sample Retrieval. Removing 1 vial from the liquid nitrogen freezer or minus 80 freezer.

Disposal with Certificate of Discard

Cost per card