SKCCC Flow/Mass Cytometry Core

Overview of Services

 

The SKCCC Flow/Mass Cytometry Core facility strives to provide technical expertise and instrumentation to enhance the quality of interdisciplinary research across JHU. Services include cell sorting, basic training in flow cytometry principles and instrument operation, panel design, and acquisition and analysis of data.

 Flow Cytometry Core

     Services Overview:

  • Consultation
    • Experimental design
    • Multicolor panel design
    • Data analysis
  • Cell Sorting (Temporarily not available)
    • up to 4-way collection of bulk populations
    • single cell sorting into 6, 24, 48, 96, and 384 well plates or onto slides  
  • Training
    • Flow cytometry principles
    • Instrument operation
    • Data Analysis Techniques
  • Contract Research
    • Custom flow cytometry assays designed and conducted by our trained staff
  • Acquisition and analysis of data

     Equipment Overview:

  • Analyzers:
    • BD Fortessa 
    • BD LSRII
    • CytoFLEX

 

Mass Cytometry Core

     Service overview: **Please note: All mass cytometry services require consultation and direct coordination with the facility personnel. Please contact Xuan Yuan and Won Jin Ho with inquiries. 

  • Consultation
    • Experimental Design
    • Panel construction and optimization
  • Services
    • Antibody customization
    • Sample staining
    • Multiplex barcoding
    • Suspension mass cytometry (CyTOF) Data acquisition
    • Imaging mass cytometry (IMC) Data acquisition
    • Data analysis (in collaboration with Bioinformatics/Biostats Core)

     Equipment Overview:

  • Fluidigm Helios™ (CyTOF)
  • Fluidigm Hyperion System™ (IMC)

 

 

 

Acknowledgement Policy

This Core is supported through the Sidney Kimmel Cancer Center National Cancer Institute P30 CA006973 Center Grant. 

Cancer Center members should reference CCSG funding (NCI CCSG P30 CA006973) on all cancer center publications where SKCCC Core services are used. 

Acknowledgement of the core facility in all publications which utilized the service of the core is customary and appreciated, as it is used as a metric in determining future funding.

Leadership

Franck Housseau, Ph.D. | Flow Core Director 

Ada Tam | Flow Core Manager 

Won Jin Ho,  MD | Mass Cytometry Core Director

Xuan Yuan,  MD | Mass Cytometry Core Manager

 Location and hours of operation 

Hours Location

10:00 AM-6:00 PM

Monday through Friday    

Except Holidays

Flow cytometry core in CRB I Room G36

Mass cytometry core in CRB I Room 223

 

Links and Resources

  1. www.bdbiosciences.com
  2. www.biolegend.com
  3. www.thermofisher.com
  4. www.miltenyibiotec.com
  5. www.bio-rad.com
  6. www.fluidigm.com
  7. www.cellsignaling.com

Contacts

Name Role Phone Email Location
Ada Tam
Research Associate, Flow Core Manager
 

 
adatam@jhmi.edu
 
CRB1 435A
 
Jay Cho
Research Technologist, Flow Core
 

 
jcho49@jhu.edu
 
CRB1 435A
 
Xuan Yuan
Mass Spec Core Manager
 
410-662-2388
 
yuanxu@jhmi.edu
 
CRB1 1m87
 

Fixed Cost Services


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