The Next Generation Sequencing Center (NGSC) is a member of the new Experimental and Computational Genomics Core (ECGC) at SKCCC. The NGSC, Microarry Core, and Bioinformatics core have come together to provide integrated services and educational appropriates for the Johns Hopkins University and cancer center members.
We want to make sure that your experiment is done well, from the design stage through sequencing and then analysis.
We try to support any research project. Some common applications include:
We can also handle a variety of purely computational requests on a fee-for-service basis.
Please got to our FAQ page for more detailed information.
While we prioritize projects from Cancer Center labs; we efficiently address projects from any other Johns Hopkins investigators.
More information about the core can be found on our website: ecgc.jhmi.edu.
Our Goals:
Innovation
Flexibility
Completeness
Personnel:
Vasan Yegnasubramanian, M.D., Ph.D., Facility Director
Sarah Wheelan, M.D., Ph.D., Bioinformatics Director
Leslie Cope, Ph.D., Bioinformatics Director
Kornel Schuebel, Ph.D., Laboratory Director
Ludmila Danilova, Ph.D.
Lauren Ciotti, Communications Associate
Jennifer Meyers, Lab Manager
Alyza Skaist, MS, Bioinformatics Analyst
Anuj Gupta, MS, Bioinformatics Analyst
Hours | Location |
8:30 - 5pm Monday - Friday Excluding Holidays |
1550 Orleans St. CRB-II, Room 131 Baltimore, Maryland 21231 |
Consultation meetings are on Friday mornings from 10AM to 12Noon in CRB1, 192.
Sample drop-off times are on Tuesday and Thursday from 10AM to 11AM and 1PM to 2PM.
Name | Role | Phone | Location | |
---|---|---|---|---|
Lauren Ciotti |
Project Coordinator
|
443-287-7341
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ECGC@jhmi.edu
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CRB2 1M50
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Service List |
► Bioinformatics (1) | |||
Name | Description | Price | |
---|---|---|---|
BioInformatics Services | Inquire | ||
► Custom Library (4) | |||
Name | Description | Price | |
Library Tier I |
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Inquire | |
Library Tier II |
|
Inquire | |
Library tier III |
|
Inquire | |
QC | Inquire | ||
► Illumina Sequencing (10) | |||
Name | Description | Price | |
A) Illumina Sequencing 200 Cycle | Inquire | ||
B) Illumina Sequencing 100 Cycle | Inquire | ||
C) Illumina Sequencing 50 Cycle | Inquire | ||
D) PPU Lab | Inquire | ||
E) CPU Lab | Inquire | ||
F) PPU Analysis | Inquire | ||
G) CPU Analysis | Inquire | ||
H) MiSeq 500 Cycle | Inquire | ||
I) MiSeq 300 Cycle | Inquire | ||
J) MiSeq 100 Cycle | Inquire | ||
► Service (12) | |||
Name | Description | Price | |
A) ChIP Seq | Inquire | ||
B) RNA-Seq Strand Specific, 1/16 slide |
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Inquire | |
C) RNA-Seq Strand Specific, 1/8 slide |
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Inquire | |
D) RNA-Seq Low-Input Non-strand specific, 1/16 slide |
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Inquire | |
Exome Sequencing |
Includes: Sure-Select Exome sequencing Library Prep, Library QC, Emulsion, WFA, Single-read Fragment Quad sequencing, Primary and Secondary analysis, Single-read fragment quad, Highly flexible Tertiary analysis. (Variant calls, flexible/custom annotation pipelines to match research goals, data visualization) |
Inquire | |
F) RNA-Seq Prokaryotic |
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Inquire | |
G) MBD Seq |
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Inquire | |
I) MBD Seq w/ reagents supplied by user |
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Inquire | |
J) Whole Genome Sequencing, 100X coverage |
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Inquire | |
K) Whole Genome Sequencing, 10-15X |
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Inquire | |
L) Data Delivery | Inquire | ||
M) Rapid Run Surcharge | Inquire |