ALERT

In observance of US Thanksgiving holiday, Agilent Cross Lab iLab Operations Software will be closed during US hours on Thursday November 23rd and Friday November 24th, 2017. EU and APAC support coverage will remain open during this time. We will resume US support hours on Monday November 27th, 2017. For urgent matters, please add "URGENT" to the ticket subject and these requests will be prioritized first.

SKCCC Cell Imaging Facility

Overview of Services

 

The Cell Imaging Core Facilities' mission has been to provide state-of-the-art cell imaging technologies to SKCCC members. Services include light and fluorescence microscopy, stereo and confocal microscopy, infrared imaging, time lapse, and laser microdissection. Images can be acquired using a variety of video and CCD cameras, and then analyzed and manipulated with cutting edge image analysis software programs. Users receive technical support by the core personnel and detailed instruction in the use of all core equipment.

 

Brightfield and fluorescent microscopy

All of the microscopes in our facility are brightfield as well as fluorescent capable. A variety of filter cubes allows the identification and visualization distinct populations of stained cells. A choice of either a high resolution black and white ccd camera or a color camera allows the acquisition of publication quality microscopic images. Comprehensive software allows for quantitative analysis.

 

 

Odyssey ®- Infrared Imaging System

With direct infrared detection, the Odyssey System quantifies low-abundance proteins. Two detection channels allow the simultaneous detection and analysis of two separate targets on the same gel. Other applications include in-cell western and tissue imaging.

 

 

Data courtesy LI-COR Biosciences

Odyssey and LI-COR are registered trademarks of LI-COR Inc., used by permission

 

Confocal Microscopy

Our most recent acquisition is the Nikon C1si True Spectral Imaging Confocal Laser Scanning Microscope System. One of the advantages of conventional Confocal microscopy is the ability to optically section tissue or cells therefore eliminating out-of-focus glare. The C1si also has the capability of acquiring 32 channels of fluorescent spectra over a 320 nm wide wavelength in a single pass using spectral imaging.

 

 

Timelapse Microscopy

The Nikon TE2000E inverted microscope is equipped with the Live Cell Incubation system, which is very stable and allows for optimally adjusted CO2 flow. Extended time lapse data can be acquired and analyzed with Elements software.

 

 

Stereoscopic Microscopy

The Nikon SMZ 1500 fluorescent stereoscopic zoom microscope is suitable for quickly assessing the presence of fluorescent proteins, such as GFP in live cells. In addition, the low magnification capability permits photography of whole tissue mounts.

 

 

Laser Capture Microdissection

The Leica LMD 7000 has proven to be a valuable asset to the Core users. With the ability to dissect out discrete cells or cellular components as well as defined areas of tissue, this apparatus provides the means for obtaining pure populations of cell types from histological sections, thereby facilitating a broad range of projects.

 

Cameras and Imaging Software

The facility provides access to four black and white high resolution CCD cameras as well as a 12-million-pixel digital Nikon DXM 1200F. The different camera types are set up to be used on any of the microscopes in the facility. Advanced software packages are available for image acquisition and analysis, ACT-1 and Elements.

 

 

Leadership

Fred Bunz, MD, PhD

Faculty Director

 

Alan Meeker, Ph.D.

Co-Director

 

Lillian Dasko-Vincent

Core Manager

ldaskov1@jhmi.edu

Location and hours of operation

Hours Location

Monday - Friday

8:30 - 4:30

Except Holidays

1650 Orleans St

CRB-1, Room G-26

Baltimore, MD 21231

Campus Map

 

 

Contacts

Name Role Phone Email Location
Lillian Dasko-Vincent
Core Manager
 
410-614-4414
 
ldaskov1@jhmi.edu
 

 



Search available services: View: by category alphabetically
Equipment Charges (1)
Software (2)
Timelapse (2)


Members at Mid-Atlantic Shared Services Consortium

Core Name Primary Contact Email Phone Number/Ext
Biostatistics and Bioinformatics Shared Service

State-of-the-art statistical science, expert statistical analysis and sound statistical reporting

Susan Holt sholt@som.umaryland.edu (410) 706-8505
Confocal Microscopy Core

Equipment and expertise to assist investigators in applying advanced fluorescence microscopy techniques to their research.

Joseph Mauban, PhD jmauban@som.umaryland.edu 410-706-6170
Core for Translational Research in Imaging (CTRIM)

State-of-the-art technology for animal imaging at the molecular, cellular and systemic level. Services for in-vivo and ex-vivo cross-sectional and functional imaging using MRI, MR-g-FUS, PET, and CT.

Jiachen Zhuo jzhuo@umm.edu (410) 328-6443
Electron Microscopy Core Imaging Facility

‌‌‌‌‌Transmission Electron Microscope, Scanning Electron Microscope, SEM CryoTransfer & Chamber, High Pressure Freezer, Freeze Substitution System, Grid Plunger, CryoUltramicrotome, Gatan 626 cryo holder

Ru-Ching Hsia, PhD rhsia@umaryland.edu 410-7067992
Flow Cytometry Core

Sample acquisition on three analytical flow cytometers, expert data analysis, experimental planning and consultation and perform cell sorting on two high speed cell sorters.</p>

Karen Underwood, BS Flowcore@som.umaryland.edu 410-706-2173
Genomics Core Facility - Formerly Biopolymer Core Facility

DNA Sequencing, Cell Line Authentication, Gene Expression Microarray, Genotyping and more

Nicholas Ambulos, PhD nambulos@som.umaryland.edu 410-706-60665
Mass Spectrometry Center

Strives to accelerate discovery by providing investigators, on campus and beyond, access to cutting-edge technologies in mass spectrometry. Expetise from basic biology and medicine to technology development and translational research

Maureen A. Kane mkane@rx.umaryland.edu 410-706-5097
Pathology Biorepository Shared Service (PBSS)

Provides the collection of high quality banked patient samples while maintaining patient confidentiality. PBSS also provides pathology, histology, & histotechnology consultation services to assist principal investigators in the procurement, analyses, & clinicopathologic correlation of human tissue

Ashley Cellini, P.A. (ASCP) acellini@som.umaryland.edu 410-706-2174
Translational Laboratory Shared Service (UMGCC)

Pre-clinical and clinical experimental support to basic researchers and physicians. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.

Rena Lapidus, PhD RLapidus@som.umaryland.edu 410-328-8092
Core Name Primary Contact Email Phone Number/Ext
Non-iLab, JHU SKCCC Core Facilities

Jeff Smith smithje@jhmi.edu
SKCCC IRAT Core (Image Response Assessment Team)

Michael Jacobs mjacobs8@jhmi.edu
SKCCC Immune Monitoring Core (formerly Human Immunology)

Christopher Thoburn cthoburn@jhmi.edu 410-955-8568
SKCCC Specimen Accessioning Core

Robin Stewart rstewa43@jhmi.edu 410 502-0674
SKCCC Analytical Pharmacology Core

Michelle Rudek Mrudek2@jhmi.edu 410-614-6321
SKCCC Animal Resources

Eric Davis edavisa@jhmi.edu 410-502-9544
SKCCC Cell Imaging Facility

Lillian Dasko-Vincent ldaskov1@jhmi.edu 410-614-4414
SKCCC Common Equipment Core

Eric Davis edavisa@jhmi.edu
SKCCC Cytogenetics Core Facility

Laura Morsberger lmorsber@jhmi.edu
SKCCC Experimental Irradiator Core

Esteban Velarde evelard2@jhmi.edu
SKCCC Flow Cytometry Core

Julie Nauroth jnaurot1@jhmi.edu 443-287-3952
SKCCC Microarray Core

Wayne Yu wyu8@jhmi.edu 410-502-7970
SKCCC Next Generation Sequencing Core

Lauren Ciotti nextgenseq@jhmi.edu 443-287-7341

University of Virginia

Core Name Primary Contact Email Phone Number/Ext
Advanced Microscopy Facility Stacey Criswell sjm7r@virginia.edu 434-924-2524
BioNMR Facility
Biomolecular Analysis Facility
Biorepository and Tissue Research Facility (BTRF) Craig Rumpel, MS crumpel@virginia.edu 434-982-6453
Flow Cytometry Core Facility FCCF Core Email fccf-staff2@virginia.edu
Genetically Engineered Murine Model (GEMM) Core
Merry X-Ray Leslie Yowell lfy8p@virginia.edu (434) 243-6051
Metabolomics Lipidomics Shared Resource Todd Fox tfox@virginia.edu 434-297-6516
MolMart & Tissue Culture Facility Linda Beggerly lkb7h@virginia.edu 434 924 9211
Molecular Electron Microscopy Core Kelly Dryden kdryden@virginia.edu 434.982.0525
Molecular Imaging Core Jack Roy jrr5a@virginia.edu (434) 243-9232
Office of Research Core Administration Alicia Johnson af7sb@virginia.edu 434-982-6565
SOM Clinical Research Unit (CRU)
Stem Cell Core Arazdordi Toumadje AT5KH@virginia.edu 434-297-5302
Surgical Skills Training Center Dana Davis Sikon dds4g@eservices.virginia.edu 434-924-6854